CHOFlow® is FyoniBio’s proprietary glycoengineered CHO cell line, developed to produce afucosylated monoclonal antibodies (mAbs) and other glycoproteins with enhanced antibody-dependent cellular cytotoxicity (ADCC). This platform is built on our robust CHOnamite® background and includes a FUT8 knock-out (KO) to eliminate core fucosylation—a critical quality attribute (CQA) that directly impacts mAb efficacy.
N-glycosylation patterns on monoclonal antibodies (mAbs) are critical quality attributes (CQAs) that affect a drug’s efficacy, safety, and pharmacokinetics. Among them, core fucosylation on the Fc region plays a particularly important role.
This sugar modification directly influences the antibody’s ability to bind to FcγRIIIa receptors on natural killer (NK) cells. Removing core fucose significantly increases binding affinity—up to 10-fold—enhancing antibody-dependent cellular cytotoxicity (ADCC), a key mechanism of action in many immunotherapies.
CHOFlow® enables this precise glyco-optimization while preserving high expression levels and robust process performance, making it an ideal platform for developing next-generation antibodies with enhanced effector functions.
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Platform Highlights
High yields > 5 g/L in standard fed-batch processes, for scalable manufacturing
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Platform Highlights
Built on the robust CHOnamite® host, with targeted FUT8 gene deletion to consistently produce afucosylated glycoproteins and boost ADCC activity
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Platform Highlights
Maintains stability over > 70 population doublings—meeting long-term GMP manufacturing standards
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Platform Highlights
Core fucosylation is removed to boost ADCC potency, while preserving other N-glycan structures critical for safety, stability, and pharmacokinetics
CHOFlow® cell lines are engineered to enhance the therapeutic profile of antibodies, particularly those relying on ADCC for their mechanism of action.
Up to 10x increased affinity
Greater NK cell-mediated cytotoxicity
May enable reduced treatment dosages
Suitable for low receptor density and low-affinity Fc receptor variants (e.g., F-variant)
Discuss your project with our team—we typically respond within 24 hours.
Each project is unique. We can tailor our services to your specific needs. Each individual project is planned to meet the customer’s specifications, costs and time lines. The project plan, consisting of one or more working packages, has a flexible design and can be discussed and adapted, if desired. Get in touch with us and discuss the specific requirements of your project.
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Illustration designer: Tilo Schneider
